toluidine blue การใช้

• Therefore, toluidine blue will appear purple to red when it stains these components.
• The permanganate-bisulfite-toluidine blue reaction is a highly selective and sensitive method for demonstrating elastic fibers under polarizing optics.
• The mechanism is different from that of nuclear staining by basic ( cationic ) dyes such as thionine or toluidine blue.
• Alkaline solutions of toluidine blue are commonly used for staining semi-thin ( 0.5 to 1 ?m ) sections of resin-embedded tissue.
• Toluidine blue staining, where the dye is preferentially retained by dysplastic tissue, is sometimes used, but there is high false positive rate.
• Indeed, Dr . Arthur Mashberg, a now retired oral cancer pioneer, first patented a toluidine blue test for the disease back in 1982.
• Metachromatic granules due to their displaying the metachromatic effect; they appear red or blue when stained with the blue dyes methylene blue or toluidine blue.
• The metachromatic properties of dimethylmethylene blue, a thiazine dye closely related to toluidine blue, have been exploited to assay glycosaminoglycans extracted from cartilage and other connective tissues.
• When these tissues are stained with a concentrated basic dye solution, such as toluidine blue, the bound dye molecules are close enough to form dimeric and polymeric aggregates.
• He and two colleagues developed a test that makes use of toluidine blue, a dye that is used to color textiles but that has long been put to medical use as well.
• Toluidine blue is an example of a partially orthochromatic dye, as it stains nucleic acids by its orthochromatic color ( blue ), but stains mast cell granules in its metachromatic color ( red ).
• Some of the dyes used are methylene blue, prussian blue, brilliant cresyl blue, Toluidine Blue O, Basic Blue 3, Methylene Green, Taylor's Blue, Janus Green B, Meldola's Blue, Thionin, Nile Blue, and Celestine Blue.
• Other methods involve the use of illuminescence, relying on either the property of normal autoflorescent molecules in mucosa such as collagen and keratin which is lost from areas of dysplasia or carcinoma under blue light, or by initially staining of the mucosa with toluidine blue or dilute acetic acid and examination under white light.